Rapid Mouse Brain Imaging Service
We prepare mouse brains through tissue clearing and sectioning, followed by VISoR 3D fluorescence imaging. Deliverables include maximum-intensity projection images. We also provide petabyte-scale data storage, management, and analysis—including image stitching, 3D reconstruction, atlas registration, and neuronal morphology reconstruction—using high-performance computing clusters and proprietary algorithms.
Supported labeling methods: transgenic labeling, viral tracing, CTB tracing, nuclear (DAPI) staining, immunofluorescence staining, and Nissl staining.
Typical turnaround time: 10 days
Preparation process
1. Sample labeling. We support transgenic labeling, viral tracing, CTB tracing, nuclear (DAPI) staining, immunofluorescence staining, and Nissl staining.
2. Brain extraction and delivery. Send us the perfused mouse-brain samples.
3. Fixation and embedding. Embed the samples in hydrogel to preserve their morphology and minimize deformation.
4. Thick sectioning. Use a vibrating microtome to cut the embedded mouse brain into 300 μm sections.
5. Slide mounting. Mount 40–50 prepared mouse-brain sections on a specialized slide for clearing.
6. Tissue clearing. Clear the tissue using the PuClear method. Small-molecule or antibody labeling, if required, is performed after clearing.
7. VISoR imaging. Acquire 3D fluorescence images of the entire mouse brain at a voxel size of 1 × 1 × 2.5 μm³ in approximately 30 minutes.
8. 2D reconstruction. Generate maximum-intensity projection images of the brain sections for rapid assessment.
9. Additional analyses. Perform 3D reconstruction, cell counting, neuronal morphology reconstruction, and more.
Sample labeling
Supports various labeling methods including transgenic labeling, viral tracing, CTb tracing, cell nucleus (DAPI) staining, immunofluorescence staining, Nissl staining, etc.
Brain extraction and delivery
Send the perfused mouse brain samples to us
Fixation and embedding
Embed the sample in hydrogel to preserve its morphology and minimize deformation.
Cut into thick slices
Use a vibrating microtome to cut the embedded mouse brain into 300-micron thick slices
Slide Mounting
Attach 40-50 prepared mouse brain slices to a special slide for clearing
Tissue clearing
Use the PuClear method for tissue clearing. If small molecules or antibody labeling is needed, it should be done after clearing.
VISoR imaging
Using the VISoR microscope to complete the 3D fluorescence imaging of the entire mouse brain within half an hour at a resolution of 1×1×2.5μm³.
2D reconstruction
Generate maximum intensity projection images of these brain slices for quick assessment of sample research value
More analyses
3D reconstruction, cell counting, neuron morphology reconstruction, etc.